ThaumatinV1, Main, Exploration, bibRecord, 000042

Gene Cloning, Expression, and Antifungal Activities of Permatin from Naked Oat (Avena nuda).

Identifieur interne : 000042 ( Main/Exploration ); précédent : 000041; suivant : 000043

Gene Cloning, Expression, and Antifungal Activities of Permatin from Naked Oat (Avena nuda).

Auteurs : Jian Liu [République populaire de Chine] ; Deping Han [République populaire de Chine] ; Yawei Shi [République populaire de Chine]

Source :

Probiotics and antimicrobial proteins [ 1867-1314 ] ; 2019.

RBID : pubmed:29717420

Descripteurs français

KwdFr :
- Alignement de séquences (MeSH), Antifongiques (composition chimique), Antifongiques (métabolisme), Antifongiques (pharmacologie), Avena (composition chimique), Avena (génétique), Avena (métabolisme), Clonage moléculaire (MeSH), Espèces réactives de l'oxygène (métabolisme), Feuilles de plante (composition chimique), Feuilles de plante (génétique), Feuilles de plante (métabolisme), Fusarium (croissance et développement), Fusarium (effets des médicaments et des substances chimiques), Fusarium (métabolisme), Masse moléculaire (MeSH), Mycelium (croissance et développement), Mycelium (effets des médicaments et des substances chimiques), Mycelium (métabolisme), Phylogenèse (MeSH), Protéines végétales (composition chimique), Protéines végétales (génétique), Protéines végétales (métabolisme), Protéines végétales (pharmacologie), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH).
MESH :
- composition chimique : Antifongiques, Avena, Feuilles de plante, Protéines végétales.
- croissance et développement : Fusarium, Mycelium.
- effets des médicaments et des substances chimiques : Fusarium, Mycelium.
- génétique : Avena, Feuilles de plante, Protéines végétales.
- métabolisme : Antifongiques, Avena, Espèces réactives de l'oxygène, Feuilles de plante, Fusarium, Mycelium, Protéines végétales.
- pharmacologie : Antifongiques, Protéines végétales.
- Alignement de séquences, Clonage moléculaire, Masse moléculaire, Phylogenèse, Séquence d'acides aminés, Séquence nucléotidique.

English descriptors

KwdEn :
- Amino Acid Sequence (MeSH), Antifungal Agents (chemistry), Antifungal Agents (metabolism), Antifungal Agents (pharmacology), Avena (chemistry), Avena (genetics), Avena (metabolism), Base Sequence (MeSH), Cloning, Molecular (MeSH), Fusarium (drug effects), Fusarium (growth & development), Fusarium (metabolism), Molecular Weight (MeSH), Mycelium (drug effects), Mycelium (growth & development), Mycelium (metabolism), Phylogeny (MeSH), Plant Leaves (chemistry), Plant Leaves (genetics), Plant Leaves (metabolism), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Proteins (pharmacology), Reactive Oxygen Species (metabolism), Sequence Alignment (MeSH).
MESH :
- chemical , chemistry : Antifungal Agents, Plant Proteins.
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Antifungal Agents, Plant Proteins, Reactive Oxygen Species.
- chemical , pharmacology : Antifungal Agents, Plant Proteins.
- chemistry : Avena, Plant Leaves.
- drug effects : Fusarium, Mycelium.
- genetics : Avena, Plant Leaves.
- growth & development : Fusarium, Mycelium.
- metabolism : Avena, Fusarium, Mycelium, Plant Leaves.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Molecular Weight, Phylogeny, Sequence Alignment.

Abstract

Thaumatin-like proteins (TLPs) are the products of a large, highly complex gene family involved in host defense. TLPs also belong to the pathogenesis-related family 5 (PR-5) of plant defense proteins. Most TLPs exhibit potential antifungal activities, and their accumulation in the plant is related to many physiological processes. In this study, a gene encoding TLP named permatin with an open reading frame of 678 bp encoding a protein of 225 amino acids with a calculated molecular mass of 23.5 kDa was cloned from naked oat leaves. Phylogenetic analysis revealed that permatin shares high homology with a number of other TLPs among diverse taxa. Model of structure by homology modeling showed that permatin consists of an acidic cleft region consistent with most TLPs. Recombinant NusA-permatin was overexpressed in Escherichia coli strain BL21 and purified by Heparin column combined with Sephacryl S-200 column. The protein exhibited antifungal activity to Fusarium oxysporum (half maximal inhibitory concentration, IC₅₀ = 21.42 μM). Morphological observation showed that NusA-permatin can induce mycelium deformation of F. oxysporum, the cell membrane is blurred, and the diaphragm is not obvious. NusA-permatin also causes membrane permeabilization and reactive oxygen species accumulation in the mycelium of F. oxysporum. Permatin may play an important role in the disease resistance responses of plants against pathogen attacks through its antifungal activity.

DOI: 10.1007/s12602-018-9422-y
PubMed: 29717420

Affiliations:

République populaire de Chine

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000063
to stream Main, to step Curation: 000063

Le document en format XML

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<term>Antifungal Agents (metabolism)</term>
<term>Antifungal Agents (pharmacology)</term>
<term>Avena (chemistry)</term>
<term>Avena (genetics)</term>
<term>Avena (metabolism)</term>
<term>Base Sequence (MeSH)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>Fusarium (drug effects)</term>
<term>Fusarium (growth & development)</term>
<term>Fusarium (metabolism)</term>
<term>Molecular Weight (MeSH)</term>
<term>Mycelium (drug effects)</term>
<term>Mycelium (growth & development)</term>
<term>Mycelium (metabolism)</term>
<term>Phylogeny (MeSH)</term>
<term>Plant Leaves (chemistry)</term>
<term>Plant Leaves (genetics)</term>
<term>Plant Leaves (metabolism)</term>
<term>Plant Proteins (chemistry)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plant Proteins (pharmacology)</term>
<term>Reactive Oxygen Species (metabolism)</term>
<term>Sequence Alignment (MeSH)</term>
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<term>Antifongiques (métabolisme)</term>
<term>Antifongiques (pharmacologie)</term>
<term>Avena (composition chimique)</term>
<term>Avena (génétique)</term>
<term>Avena (métabolisme)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Espèces réactives de l'oxygène (métabolisme)</term>
<term>Feuilles de plante (composition chimique)</term>
<term>Feuilles de plante (génétique)</term>
<term>Feuilles de plante (métabolisme)</term>
<term>Fusarium (croissance et développement)</term>
<term>Fusarium (effets des médicaments et des substances chimiques)</term>
<term>Fusarium (métabolisme)</term>
<term>Masse moléculaire (MeSH)</term>
<term>Mycelium (croissance et développement)</term>
<term>Mycelium (effets des médicaments et des substances chimiques)</term>
<term>Mycelium (métabolisme)</term>
<term>Phylogenèse (MeSH)</term>
<term>Protéines végétales (composition chimique)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Protéines végétales (pharmacologie)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antifungal Agents</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term>
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<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Antifongiques</term>
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<term>Feuilles de plante</term>
<term>Protéines végétales</term>
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<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Fusarium</term>
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<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Fusarium</term>
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<term>Mycelium</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Avena</term>
<term>Plant Leaves</term>
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<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Fusarium</term>
<term>Mycelium</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Avena</term>
<term>Feuilles de plante</term>
<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Avena</term>
<term>Fusarium</term>
<term>Mycelium</term>
<term>Plant Leaves</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antifongiques</term>
<term>Avena</term>
<term>Espèces réactives de l'oxygène</term>
<term>Feuilles de plante</term>
<term>Fusarium</term>
<term>Mycelium</term>
<term>Protéines végétales</term>
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<term>Sequence Alignment</term>
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<term>Masse moléculaire</term>
<term>Phylogenèse</term>
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<front><div type="abstract" xml:lang="en">Thaumatin-like proteins (TLPs) are the products of a large, highly complex gene family involved in host defense. TLPs also belong to the pathogenesis-related family 5 (PR-5) of plant defense proteins. Most TLPs exhibit potential antifungal activities, and their accumulation in the plant is related to many physiological processes. In this study, a gene encoding TLP named permatin with an open reading frame of 678 bp encoding a protein of 225 amino acids with a calculated molecular mass of 23.5 kDa was cloned from naked oat leaves. Phylogenetic analysis revealed that permatin shares high homology with a number of other TLPs among diverse taxa. Model of structure by homology modeling showed that permatin consists of an acidic cleft region consistent with most TLPs. Recombinant NusA-permatin was overexpressed in Escherichia coli strain BL21 and purified by Heparin column combined with Sephacryl S-200 column. The protein exhibited antifungal activity to Fusarium oxysporum (half maximal inhibitory concentration, IC<sub>50</sub>
 = 21.42 μM). Morphological observation showed that NusA-permatin can induce mycelium deformation of F. oxysporum, the cell membrane is blurred, and the diaphragm is not obvious. NusA-permatin also causes membrane permeabilization and reactive oxygen species accumulation in the mycelium of F. oxysporum. Permatin may play an important role in the disease resistance responses of plants against pathogen attacks through its antifungal activity.</div>
</front>
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<DateCompleted><Year>2019</Year>
<Month>08</Month>
<Day>13</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>09</Month>
<Day>16</Day>
</DateRevised>
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<JournalIssue CitedMedium="Internet"><Volume>11</Volume>
<Issue>1</Issue>
<PubDate><Year>2019</Year>
<Month>03</Month>
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<Title>Probiotics and antimicrobial proteins</Title>
<ISOAbbreviation>Probiotics Antimicrob Proteins</ISOAbbreviation>
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<ArticleTitle>Gene Cloning, Expression, and Antifungal Activities of Permatin from Naked Oat (Avena nuda).</ArticleTitle>
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</Pagination>
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<Abstract><AbstractText>Thaumatin-like proteins (TLPs) are the products of a large, highly complex gene family involved in host defense. TLPs also belong to the pathogenesis-related family 5 (PR-5) of plant defense proteins. Most TLPs exhibit potential antifungal activities, and their accumulation in the plant is related to many physiological processes. In this study, a gene encoding TLP named permatin with an open reading frame of 678 bp encoding a protein of 225 amino acids with a calculated molecular mass of 23.5 kDa was cloned from naked oat leaves. Phylogenetic analysis revealed that permatin shares high homology with a number of other TLPs among diverse taxa. Model of structure by homology modeling showed that permatin consists of an acidic cleft region consistent with most TLPs. Recombinant NusA-permatin was overexpressed in Escherichia coli strain BL21 and purified by Heparin column combined with Sephacryl S-200 column. The protein exhibited antifungal activity to Fusarium oxysporum (half maximal inhibitory concentration, IC<sub>50</sub>
 = 21.42 μM). Morphological observation showed that NusA-permatin can induce mycelium deformation of F. oxysporum, the cell membrane is blurred, and the diaphragm is not obvious. NusA-permatin also causes membrane permeabilization and reactive oxygen species accumulation in the mycelium of F. oxysporum. Permatin may play an important role in the disease resistance responses of plants against pathogen attacks through its antifungal activity.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName>
<ForeName>Jian</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, People's Republic of China.</Affiliation>
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<Author ValidYN="Y"><LastName>Han</LastName>
<ForeName>Deping</ForeName>
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<AffiliationInfo><Affiliation>Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, People's Republic of China.</Affiliation>
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<Author ValidYN="Y"><LastName>Shi</LastName>
<ForeName>Yawei</ForeName>
<Initials>Y</Initials>
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<AffiliationInfo><Affiliation>Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, People's Republic of China. yaweishi@sxu.edu.cn.</Affiliation>
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<Agency>Key Research and Development Program of Shanxi Province</Agency>
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<Grant><GrantID>No2017019</GrantID>
<Agency>Shanxi Scholarship Council of China</Agency>
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<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
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<MeshHeading><DescriptorName UI="D018554" MajorTopicYN="N">Avena</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
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<MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName>
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<MeshHeading><DescriptorName UI="D003001" MajorTopicYN="Y">Cloning, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005670" MajorTopicYN="N">Fusarium</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008970" MajorTopicYN="N">Molecular Weight</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D025282" MajorTopicYN="N">Mycelium</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Antifungal activity</Keyword>
<Keyword MajorTopicYN="Y">Fusarium oxysporum</Keyword>
<Keyword MajorTopicYN="Y">Gene cloning</Keyword>
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Gene Cloning, Expression, and Antifungal Activities of Permatin from Naked Oat (Avena nuda).

Gene Cloning, Expression, and Antifungal Activities of Permatin from Naked Oat (Avena nuda).

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